nPKCε, a P2Y2-R downstream effector in regulated mucin secretion from airway goblet cells
نویسندگان
چکیده
Camille Ehre, Yunxiang Zhu, Lubna H. Abdullah, John Olsen, Keiichi I. Nakayama, Keiko Nakayama, Robert O. Messing, and C. William Davis Cystic Fibrosis/Pulmonary Research and Treatment Center, Department of Cell and Molecular Physiology, University of North Carolina, Chapel Hill, North Carolina; Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoda, Japan; and Ernest Gallo Clinic & Research Center, University of California-San Franciso, San Francisco, California
منابع مشابه
Mucin secretion and PKC isoforms in SPOC1 goblet cells: differential activation by purinergic agonist and PMA.
SPOC1 cells, which are a mucin-secreting model of rat airway goblet cells, possess a luminal P2Y2 purinoceptor through which UTP, ATP, and ATPgammaS stimulate secretion with EC50 values of approximately 3 microM. PMA elicits mucin secretion with high EC50 (75 nM) and saturation (300 nM) values. For the first time in airway mucin-secreting cells, the PKC isoforms expressed and activated by a sec...
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Scott, C. E., Lubna H. Abdullah, and C. William Davis. Ca21 and protein kinase C activation of mucin granule exocytosis in permeabilized SPOC1 cells. Am. J. Physiol. 275 (Cell Physiol. 44): C285–C292, 1998.—Mucin secretion by airway goblet cells is under the control of apical P2Y2, phospholipase C-coupled purinergic receptors. In SPOC1 cells, the mobilization of intracellular Ca21 by ionomycin ...
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Mucin secretion by airway goblet cells is under the control of apical P2Y2, phospholipase C-coupled purinergic receptors. In SPOC1 cells, the mobilization of intracellular Ca2+ by ionomycin or the activation of protein kinase C (PKC) by phorbol 12-myristate 13-acetate (PMA) stimulates mucin secretion in a fully additive fashion [L. H. Abdullah, J. D. Conway, J. A. Cohn, and C. W. Davis. Am. J. ...
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